Wizard PCR Preps DNA Purification Resin from Promega

Product Specs
ItemWizard PCR Preps DNA Purification Resin
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA7181
Quantity250ml
SourceReaction, Gel
Nucleic AcidDNA Fragment, PCR Product
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Lower viral evolutionary pressure under stable versus fluctuating conditions in subzero Arctic brinesMicrobiomeAugust 7, 2023Zhi-Ping Zhong, Dean Vik, Josephine Z. Rapp, Olivier Zablocki, Heather Maughan, Ben Temperton, Jody W. Deming, Matthew B. Sullivanbuffer. DNA was extracted using Wizard® PCR Preps DNA Purification Resin and Minicolumns (Cat. No. A7181 and A7211, respectively; Promega, USA) [70]. Short- and long-read virome sequencing Following DNA

(2) Polinton-like viruses are abundant in aquatic ecosystemsMicrobiomeDecember 1, 2021Christopher M. Bellas, Ruben SommarugaPromega Wizard® Minicolumns per replicate to extract DNA with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170), and the DNA was eluted from each column using 200 μL of 1 × TE buffer (pH 8). The

(3) Glacier ice archives nearly 15,000-year-old microbes and phagesMicrobiomeDecember 1, 2021Zhi-Ping Zhong et al.genomic DNA was then extracted using Wizard® PCR Preps DNA Purification Resin and Minicolumns (Cat. No. A7181 and A7211, respectively; Promega, USA) [45]. Viral abundance, calculated prior to DNA extraction, was

(4) Induction and Regulation of the Immunoproteasome Subunit β5i (PSMB8) in Laryngeal and Hypopharyngeal Carcinoma CellsMedical Science MonitorJuly 7, 2020Nan-Xiang Chen, Kun Liu, Xuan Liu, Xin-Xin Zhang, Dong-yi Hanladder, 26616) (Thermo Scientific); GelRed nucleic acid dye (BIOTIUM); DNA fragment extraction kit (A7181, column centrifugation) and PCR product purification kit (Promega); plasmid extraction kit and RNA extraction

(5) Viral Ecogenomics of Arctic Cryopeg Brine and Sea IcemSystemsJune 30, 2020Zhi-Ping Zhong et al.2a01:4f9:3a:1a85::2. MATERIALS AND METHODS using Wizard PCR Prep DNA puriﬁcation resin and minicolumns (no. A7181 and A7211, respectively; Promega, USA) (34). The DNA libraries were prepared using the Accel-NGS 1S Plus DNA library kit

(6) Towards optimized viral metagenomes for double-stranded and single-stranded DNA viruses from challenging soilsPeerJJuly 4, 2019Gareth Trubl, Simon Roux, Natalie Solonenko, Yueh-Fen Li, Benjamin Bolduc, Josué Rodríguez-Ramos, Emiley A. Eloe-Fadrosh, Virginia I. Rich, Matthew B. Sullivanelution volume) using one of three methods: Wizard mini columns (Wizard; Promega, Madison, WI, products A7181 and A7211), cetyl trimethylammonium bromide (CTAB; Porebski, Bailey & Baum, 1997), or modified DNeasy

(7) Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 InfectionAntibiotics (Basel)November 16, 2018Cristina Howard-Varona et al.DNase. After the DNA was inactivated, extraction was continued using a Wizard DNA Clean-up Kit (Cat. #A7181, Promega Corporation, Madison, WI, USA). Then, 1 mL of DNA clean-up resin was added to each sample,

(8) Soil Viruses Are Underexplored Players in Ecosystem Carbon ProcessingmSystemsOctober 30, 2018Gareth Trubl et al.to reference 50). The viral DNA was extracted using Wizard columns (Promega, Madison, WI; products A7181 and A7211), cleaned up with AMPure beads (Beckman Coulter, September/October 2018 Volume 3 Issue 5 e00076-18 msystems.asm.org

(9) Host-linked soil viral ecology along a permafrost thaw gradientNature MicrobiologyAugust 1, 2018Joanne B. Emerson et al.purification17, viral DNA was extracted from each of the seven subsamples, using Wizard columns and resin (A7181 and A7211, Promega), cleaned with AMPure beads (A63881, Beckman Coulter) and concentrated with Zymo

(10) Ocean plankton. Patterns and ecological drivers of ocean viral communitiesScienceMay 22, 2015Jennifer R. Brum et al.activity, and extracted as previously described (14). Briefly, viral particle suspensions were treated with Wizard PCR Preps DNA Purification Resin (Promega, WI, USA) at a ratio of 0.5 mL sample to 1 mL resin, and eluted with TE buffer (10 mM Tris,

(11) Life-Style and Genome Structure of Marine Pseudoalteromonas Siphovirus B8b Isolated from the Northwestern Mediterranean SeaPLOS ONEJanuary 14, 2015Elena Lara, Karin Holmfeldt, Natalie Solonenko, Elisabet Laia Sà, J. Cesar Ignacio-Espinoza, Francisco M. Cornejo-Castillo, Nathan C. Verberkmoes, Dolors Vaqué, Matthew B. Sullivan, Silvia G. Acinasglycol as described earlier (CsCl purification section). One ml of Purification Resin (Promega, product A7181 Madison WI) was added to 1.5 ml of phages (the PEG pellet resuspended with MSM) and mixed gently by

(12) Sequencing platform and library preparation choices impact viral metagenomesBMC GenomicsDecember 1, 2013Sergei A Solonenko, J César Ignacio-Espinoza, Adriana Alberti, Corinne Cruaud, Steven Hallam, Kostas Konstantinidis, Gene Tyson, Patrick Wincker, Matthew B Sullivanparticles. DNA was extracted using the Wizard Prep DNA Purification system (Promega, cat# A7211 and A7181). DNA Source for TARA metagenomes 20—60L seawater was collected and filtered for two TARA Oceans [21]

(13) Evaluation of methods to concentrate and purify ocean virus communities through comparative, replicated metagenomicsEnvironmental MicrobiologyMay 1, 2013Bonnie L. Hurwitz, Li Deng, Bonnie T. Poulos, Matthew B. Sullivanparticles using Wizard® PCR Preps DNA Purification Resin and Minicolumns (Promega, Madison, WI, USA; Cat. #A7181 and A7211 respectively) as previously described (Henn et al., 2010). The DNA was prepared for sequencing

(14) Analysis of High-Throughput Sequencing and Annotation Strategies for Phage GenomesPLoS ONEFebruary 5, 2010Matthew R. Henn et al.(pH 7.4), 10 mM MgSO4), and transferred to a 2-ml Eppendorf tube. One ml Purification Resin (product A7181, Promega, Madison WI) was then added and mixed gently by inverting the tube. The resulting slurry was

(15) The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 proteinNucleic Acids ResearchNovember 1, 2007Marc Abella, Sonia Rodríguez, Sonia Paytubi, Susana Campoy, Malcolm F. White, Jordi Barbéelectrophoresis in a 2–3% low-melting-point agarose gel was performed and the PCR amplicons were purified using Wizard PCR preps DNA purification Resin (Promega). Binding reactions (20 μl) containing 20 ng of the DIG-DNA-labelled probe and 1 μl of S. solfataricus

(16) Single-molecule and population probing of chromatin structure using DNA methyltransferasesMethodsMarch 1, 2007Jessica A KILGORE, Scott A HOOSE, Tanya L GUSTAFSON, Weston PORTER, Michael P KLADDEgloves and safety glasses. Dispose of solutions properly. 8. Promega Wizard PCR Preps resin (cat. #A7181). Caution: The resin contains 6 M guanidine thiocyanate, which is an irritant. 9. Promega Wizard minicolumns

(17) Significant association of interleukin 8 −251T/A polymorphism with smoking behavior in a Japanese populationJournal of Human GeneticsNovember 1, 2005Hidemi Ito, Keitaro Matsuo, Nobuyuki Hamajima, Katashi Okuma, Toshiko Saito, Kazuo Tajimaverify the sizes. After standard procedures of puriﬁcation (Wizard PCR Preps DNA Puriﬁcation Resin, Cat# A7181, Promega) and extraction with phenol chloroform and ethanol, all the PCR products were subjected to

(18) Association study between the IL4, IL13, IRF1 and UGRP1 genes in chromosomal 5q31 region and Chinese Graves’ diseaseJournal of Human GeneticsNovember 1, 2005Yu Yang, Sun Lingling, Jin Ying, Li Yushu, Shan Zhongyan, Huang Wei, Teng Weipingverify the sizes. After standard procedures of puriﬁcation (Wizard PCR Preps DNA Puriﬁcation Resin, Cat# A7181, Promega) and extraction with phenol chloroform and ethanol, all the PCR products were subjected to

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